Time: Monday 30 June, 2014 at 14:00
Place: University of Eastern Finland, Kuopio Campus, Snellmania building, room S25 address: Yliopistonranta 1 E
Lecturer: Dr. Kaisa Kajala, Postdoctoral Fellow, Brady Lab, Department of Plant Biology and Genome Center, University of California, Davis CA, U.S.A
Topic: A rapid method for translating molecular tools for crop species
Abstract: Translating existing molecular tools into crop species is fundamentally important for expanding our understanding of plant development and stress responses to crop species. However, generating transgenic lines of non-model species can be labour-intensive and take a long time. We have used Agrobacterium rhizogenes-mediated hairy root transformation to rapidly generate transgenic roots in order to optimize a range of molecular tools in tomato. We assessed gene expression patterns in tomato hairy roots, showing that the expression patterns in A. rhizogenes-transformed hairy roots are indistinguishable from roots transformed with A. tumefaciens and generating a toolbox of cell- and tissue-type specific promoters for tomato roots. We optimized two molecular methods for cell-type specific gene expression studies in tomato: Isolation of Nuclei Tagged in specific Cell-Types (INTACT) and Translating Ribosome Affinity Purification (TRAP). Finally, we tested gene function in tomato using CRISPR/Cas9 genome editing and translational reporters. Furthermore, we have used the hairy root method to generate transgenic roots in wild tomato Solanum pennelii as well as Lepidium hyssopifolium and Arabidopsis.
Publishing year: 2014Back to this years article listing