11.3. Spectroscopic and Microscopic Analysis of Immature and Mature Articular Cartilage

Public examination of a doctoral dissertation in the field of medicine

Doctoral candidate: Lic Med Jarno Rieppo

Time and venue 11.3.2011 at 13, Snellmania L22, Kuopio Campus

Osteoarthritis (OA) is the most common joint disease affecting most people over 65 years of age. In spite of intensive research work, many aspects of the biology of articular cartilage and the pathogenesis of OA have remained unknown. The postnatal development of articular cartilage has been inadequately characterized. In particular, it has been proved challenging to characterize the architecture of collagen network and compositional changes in the tissue with imaging techniques.

Thus one goal of this thesis was to develop further the methodological aspects of polarized light microscopy and Fourier transform infrared imaging spectroscopy (FT-IRIS) for use in cartilage research. This thesis work demonstrated that modern quantitative microscopy is able to measure local tissue changes that cannot be revealed with traditional biochemical techniques. When combined, polarized light microscopy and FT-IRIS provide a method for detailed characterization of collagen network architecture and volumetric changes in the amount of collagen. In particular, FT-IRIS holds a major potential for producing image maps of articular cartilage composition. However, further parameter development will have to be undertaken to increase the specificity of the FT-IRIS -derived proteoglycan and collagen parameters.

The results also demonstrate that the collagen network undergoes significant postnatal modifications. This is a further support for the concept that the quality of articular cartilage can be affected by physical exercise in childhood.

Lääketieteen lisensiaatti Jarno Riepon Itä-Suomen yliopiston biolääketieteen laitokselta valmistuva väitöskirja: ” Spectroscopic and Microscopic Analysis of Immature and Mature Articular Cartilage” (Nivelruston kollageenisäikeistössä tapahtuvat muutokset kasvun aikana: Mikroskooppinen ja spektroskooppinen tarkastelu) tarkastetaan terveystieteiden tiedekunnassa. Vastaväittäjänä toimii Professori ja kustoksena emeritusprofessori Heikki J. Helminen Itä-Suomen yliopistosta.

The doctoral dissertation of Licenciate of Medicine Jarno Rieppo, entitled Spectroscopic and Microscopic Analysis of Immature and Mature Articular Cartilage will be examined at the Faculty of Health Sciences. The opponent in the public examination will be Professor Nancy Pleshko, Philadelphia, USA, and the custos will be Professor Heikki J. Helminen of the University of Eastern Finland.

Photo available for download at http://www.uef.fi/uef/vaitoskuvat

Contact: Jarno Rieppo, tel. 040 3584948, jarno.rieppo@uef.fi

Publishing year: 2011

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